KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-DNM1 | Novus Biologicals | Cat#: NBP2-48950 |
| Rabbit polyclonal anti-KCNQ4 | Sigma-Aldrich | Cat#: HPA018305; RRID:AB_1855556 |
| Rabbit polyclonal anti-CBX3 | Novus Biologicals | Cat#: NBP1-83228; RRID:AB_11037071 |
| Rabbit polyclonal anti-SLC7A14 | Sigma-Aldrich | Cat#: HPA045929; RRID:AB_2679501 |
| Rabbit polyclonal anti-SLC26A5 | Dr. Jing Zheng, Northwestern University | Dalllos et al., Neuron 58 (3): 333-339, 2008. |
| Mouse monoclonal anti-DNM3 | Dr. Pietro De Camilli, Yale University | Raimondi et al., Neuron 70(6): 1100-1114, 2011. |
| Rabbit polyclonal anti-MYO7A | Proteus Biosciences | Cat# 25-6790; RRID: AB10015251 |
| Goat anti-rabbit antibodies (secondary) | ThermoFisher | Cat#: A-11035; RRID:AB_2534093 |
| Chemicals, peptides, and recombinant proteins | ||
| RNAscope™ 2.5 HD Assay | Advanced Cell Diagnostics | Cat#: 322360 |
| SMART-Seq v4 Ultra Low Input RNA Kit | TakaRa Bio USA Inc | Cat #: 634896, Lot#1712705A |
| Nextera Library preparation kit | Illumina | Cat#:15028211 |
| RNAlater | Thermo Fisher Scientific | Cat#: AM7020 |
| Collagenase IV | Sigma-Aldrich | Cat#: C5138 |
| Leibovitz’s L-15 | Sigma-Aldrich | Cat#: L1518 |
| Critical commercial assays | ||
| RNAscope® Probe- Mm-Sod1 | Advanced Cell Diagnostics | Cat#: 428581 |
| RNAscope® Probe- Mm-Kcnq4 | Advanced Cell Diagnostics | Cat#: 472271 |
| RNAscope® Probe- Mm-Slc17a8 | Advanced Cell Diagnostics | Cat#: 431261 |
| RNAscope® Probe- Mm-C1ql1-C2 | Advanced Cell Diagnostics | Cat#: 465081-C2 |
| RNAscope® Probe- Mm-Tmc1 | Advanced Cell Diagnostics | Cat#: 520911 |
| RNAscope® Probe- Mm-Slc7a14 | Advanced Cell Diagnostics | Cat#: 544781 |
| RNAscope® Probe- Mm-Otof | Advanced Cell Diagnostics | Cat#: 485671 |
| RNAscope® Probe- Mm-Chrna9 | Advanced Cell Diagnostics | Cat#: 525921 |
| RNAscope® Probe- Mm-Chrna10 | Advanced Cell Diagnostics | Cat#: 525911 |
| RNAscope® Probe- Mm-Clu | Advanced Cell Diagnostics | Cat#: 427891 |
| RNAscope® Probe- Mm-Foxo3 | Advanced Cell Diagnostics | Cat#: 485321 |
| RNAscope® Probe- Mm-Jund-C1 | Advanced Cell Diagnostics | Cat#: 1031951 |
| Deposited data | ||
| Hair cell transcriptomes (two time points: 9 and 26 months old) | This paper | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE153882 |
| Transcriptomes of cochlear stria vascularis (two time points: 9 months and 26 months) | This paper | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE154833 |
| Experimental models: Organisms/strains | ||
| Mouse, CBA/J | The Jackson Laboratory | www.jax.org |
| Software and algorithms | ||
| CLC Genomics Workbench software | Qiagen CLC Genomics | https://digitalinsights.qiagen.com |
| iDEP 0.93 | http://bioinformatics.sdstate.edu/idep/ | http://bioinformatics.sdstate.edu/idep/ |
| ClueGO v. 2.5.7 | Cytoscape | https://apps.cytoscape.org/apps/cluego |
| FastQC | https://basespace.illumina.com/home/index | https://basespace.illumina.com/home/index |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Adobe Photoshop and Illustrator | Adobe | https://www.adobe.com/ |
| Imaris | Oxford Instruments | https://imaris.oxinst.com/ |
| pClamp v. 10.2 | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system |
| jClamp 11.1 | SciSoft Company | http://www.scisoftco.com/ |
| Other | ||
| Ensembl database | http://uswest.ensembl.org/Mus_musculus/Info/Index | http://uswest.ensembl.org/Mus_musculus/Info/Index |
| AmiGO | http://amigo.geneontology.org/amigo | http://amigo.geneontology.org/amigo |
| gEAR | www.umgear.org | www.umgear.org |
| HGNC database | https://www.genenames.org/ | https://www.genenames.org/ |