Table 3.
Genomic position and primer sequences for the qRT-PCR assay of the 14 hypothetical and the aspC genes of the symbiosis island of Bradyrhizobium japonicum SEMIA 5079 (= CPAC 15) selected for this study
| Locus | Protein ID | Physical Location | Primer Sequence ( 5’– 3’) | Amplicon bp size | |
|---|---|---|---|---|---|
| bjs_07536 | AHY48760 | 415,677.415,677 (+) |
F GTGTCGACCAACATTCATGC R TGGGATACAGCCACGAAAC |
142 bp | |
| bjs_07605 | AHY48686 | 318,489.320,720 (+) |
F GGCAAGTTGGGTTCAGTTTG R CTTTGCGAACCGGTTATAGG |
97 bp | |
| bjs_07621 | AHY48670 | 287,116.288,339 (+) |
F GCGGCGCTATCAAATTCTAC R ACGAGCAAGTGGACAAATCC |
100 bp | |
| bjs_07647 | AHY48641 | 259,705.260,430 (-) |
F TTTCGTCATGCGTTGTGG R ATTTCCGTCCTCATGCGTAG |
114 bp | |
| bjs_08160 | AHY56892 | 9,349,925.9,350,872 (+) |
F CGATCCGCGATATCTCATTC R ATCGCGACCTTGTTCATCTC |
111 bp | |
| bjs_08216 | AHY48554 | 152,594.154,168 (+) |
F GATCCACGGGATGTTTGAAG R CGCAGATCAATTCACGTAGC |
137 bp | |
| bjs_08240 | AHY48531 | 125,139.126,544 (-) |
F CCTTCGAGGACTTGATTTCG R GACGTGTCCGTTGAATTCTG |
107 bp | |
| bjs_08251 | AHY48520 | 98,439.100,538 (+) |
F CAACGATGATACGGTTGCAG R GCATGACCTTGATTGCCTTC |
131 bp | |
| bjs_08254 | AHY48517 | 96,080.96,979 (+) |
F GGTCGGCTATTTCAAGATCG R GATAGCCCGAAATGTTGACG |
74 bp | |
|
bjs_08258 (aspC) |
AHY48514 | 91,505.92,803 (+) |
F ATCCGAGCTGCCATTACATC R GCAATGTTGTTCGGCTGTAG |
91 bp | |
| bjs_08261 | AHY48511 | 85,326.86,360 (+) |
F CGTGATCAATCTCGTCTTCG R TGAAGGAGACTTTCCGGATG |
83 bp | |
| bjs_08267 | AHY48505 | 76,029.77,225 (+) |
F TCCTGATCTGGATGTCAACG R AGAAGCATGGGATGAGCAAC |
105 bp | |
| bjs_08317 | AHY48455 | 18,261.20,504 (+) |
F AAATAGTCCGGCTGACAAGG R CATAGGTCTTCGTGCAATCG |
102 bp | |
| bjs_08523 | AHY56944 | 9,425,196.9,426,470 (+) |
F TGAGAAGCAGGAAGAGTTCG R CTCCGAAGGCGAGAAATATG |
134 bp | |
| bjs_08774 | AHY56918 | 9,392,008.9,393,012 (+) |
F ACAAAGGGTGGCTCGAAAC R TCGTAAGCGCGTAGTAAACG |
138 bp | |
(+) strand positive, (-) strand negative, F primer forward, R primer reverse