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. 2022 May 5;41:165. doi: 10.1186/s13046-022-02368-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The expression and characteristics of circ_0087429 in cervical cancer. a. Differentially expressed circRNAs between cervical cancer and adjacent tissue samples in the GSE102686 dataset. b. Differentially expressed circRNAs between cervical cancer and normal cervical epithelial cell lines in the GSE103696 dataset. c. Heatmap of circRNAs with significant differential expression in two datasets (GSE102686 and GSE103696). circ_0087429 (hsa_circRNA_104814) is marked with a red box. d. Differential expression of circ_0087429 in 44 pairs of cervical cancer and adjacent tissue samples. e. Differential expression of circ_0087429 between cervical cancer and normal cervical epithelial cell lines. f. The Sanger sequencing results showed that circ_0087429 was formed by head-to-tail splicing of the second exon of the parental gene SPIN1. g. The expression of circ_0087429 and SPIN1 after RNaseR treatment was detected by qRT-PCR. h. The expression changes in circ_0087429 and SPIN1 at different time points after adding actinomycin D were determined by qRT-PCR. i. A subcellular fractionation assay was used to detect the relative expression of circ_0087429 in the cytoplasm and nucleus. U6 was used as the internal reference for the nuclear fraction, and GAPDH was used as that for the cytoplasmic fraction. j. FISH staining confirmed the expression of circ_0087429 in the cytoplasm. Scale bar, 100 μm. ***p < 0.001