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. 2022 May 5;23(Suppl 1):346. doi: 10.1186/s12864-022-08549-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Pollen viability and morphology of tomato plants in different SlAMS modification modes. a. PCR Identification of positive plants transformed with the pCRISPR/Cas9-SlAMS vector. Lanes 1–38: PCR products of Npt II; Lane B: no target DNA; Lane N: nontransgenic plant; Lane P: pCRISPR/Cas9-SlAMS plasmid. b. Pollen viability test after SlAMS knockout (CR-T0–2) and overexpression (OV-T0–10). Blue-stained pollen grains are non-viable; colorless pollen grains are viable. c. Scanning electron microscope (SEM) images of tomato pollen grains. Wild type (WT, oval shape); pCRISPR/Cas9-SlAMS (showing shrinkage and diamond-like shapes, from CR-T0–2). pCAMBIA2301-SlAMS (showing shriveled and atrophic shapes, from OV-T0–10); d. Target site mutation examination in CRISPR/Cas9-mediated SlAMS plant. Target shows the editing site sequence. PAM indicates the adjacent motif of the protospacer sequence. CR-ams1 indicated the first type of mutation; CR-ams2 indicated the second type; CR-ams3 indicated the third type