Fig. 3. The structural basis of the hyperthermostability of SSFH-2. (a) DSC curves of SSFH-2 and its EP-deleted mutant SSFH-2-ΔEP, at scan rate of 10 °C min−1. (b) The thermal stability of SSFH-2 as a function of treatment time at fixed temperature 100 °C. (c) The crystal structure of SSFH-2 upon treated at 100 °C for 5 min, which is viewed down the 4-fold channels (PDB ID: 6J4M). (d) The position of newly formed noncovalent interactions upon thermal treatment. (e–g) Superposition of untreated (pink) and thermal-treated (blue) SSFH-2 subunit revealed the formation of several new types of new noncovalent interactions upon treated at 100 °C for 5 min.
