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. 2022 Apr 5;12:841138. doi: 10.3389/fcimb.2022.841138

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Copyright © 2022 Ashton, Sang, Blythe, Zadik, Holmes, Malla, Camps, Wright, Melchers, Verweij and Dyer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Itraconazole ETEST sensitivity testing of Aspergillus fumigatus strains 47-308 (A) and 47-51 (B) and progeny from back crossing. The ETEST has a concentration of 32 mg L^-1 itraconazole at the top of the strip which decreases down the strip towards the bottom. (A) Isolate 47-308 shows full growth over the range of itraconzole concentrations, indicating a resistance MIC of >32 mg L^-1. (B) Isolate 47-51 shows inhibition of growth at itraconazole concentrations above 0.75 mg L^-1 indicating a sensitive phenotype. (C) Representative ETEST results of progeny isolated from the third backcross between a resistant progeny isolate and the itraconazole sensitive parental isolate 47-51. Results demonstrate a 1:1 segregation of the resistant (top row) and sensitive (bottom row) phenotype.