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. 2022 Apr 5;12:841138. doi: 10.3389/fcimb.2022.841138

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Copyright © 2022 Ashton, Sang, Blythe, Zadik, Holmes, Malla, Camps, Wright, Melchers, Verweij and Dyer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Methodology of bulk segregant analysis used in the present study. An azole resistant isolate (47-308) carrying an unknown mutation was sexually crossed with a sensitive isolate (47-51). F1 progeny were collected and up to six rounds of backcrossing with the sensitive parent undertaken with arising resistant progeny. At certain stages the collected progeny were separated into azole resistant and sensitive pools, which were genome sequenced in order to identify genomic regions within the resistant progeny containing a candidate gene(s) of interest.