Table 1.

Summary of A. fumigatus bulk segregant analysis (BSA) for identification of the genetic basis of azole resistance in isolate 47-308.

Stage that BSA undertaken	Number of progeny used in each BSA pool	Number of variant genes identified	Number of contigs with SNP variants	Number of SNP variants	Sequencing depth of progeny pools
F1	40	123	11	561	124.4-126.8
Backcross 3	40	46	6	493	83.9-96.7
Backcross 6	40	22	6	135	145.8-193.5
Backcross 2	80	29	7	170	103.1-211.2

BSA was undertaken with different combinations of progeny numbers and at different stages of crossing between sensitive and resistant parents or backcrossed progeny. Results refer to SNP differences between the azole resistant pool and parent when compared to the azole sensitive pool and parent using the A1163 JGI reference genome for bioinformatic analysis.