TABLE 1.
PCR primer pairs used to amplify a portion of H. pylori genes
| Primer pair (forward primer/ reverse primer) | Encoded protein (gene [genome sequence designation]) | Nucleotide sequencea (5′→3′) | Size (bp) of PCR fragment |
|---|---|---|---|
| ERD-1/BRD-3 | Oxygen-insensitive NADPH nitroreductase (rdxA [HP0954]) | GTTAGGGATTTTATTGTATG | 427 |
| ACGCCAAGCATTTGAGCAAA | |||
| ERD-2/BRD-4 | Oxygen-insensitive NADPH nitroreductase (rdxA [HP0954]) | AAAGTTAGAGTGATCCCCTC | 351 |
| TAATTTAGGTTTGATTATAG | |||
| EFR-1/BFR-3 | NADPH flavin oxidoreductase (frxA [HP0642]) | TCTCAAGCGGAAAAATCCGG | 445 |
| AATTTTTGATGATTTGAGCG | |||
| EFR-2/BFR-4 | NADPH flavin oxidoreductase (frxA [HP0642]) | ATTATGACACTAATTCTAGG | 388 |
| CTAACGCCAAGCTTTTTATG |
a
Nucleotide sequences were obtained from the H. pylori complete genome sequence (26).