Table 1:
Key resources
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | w1118 | |||
| Genetic reagent (D. melanogaster) | zyd 1 | (Melom & Littleton, 2013) | Zyd | |
| Genetic reagent (D. melanogaster) | repo-Gal4 | (Lee & Jones, 2005) | ||
| Genetic reagent (D. melanogaster) | GMR85G01-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_40436 | Perineurial glia |
| Genetic reagent (D. melanogaster) | GMR54C07-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_50472 | Subperineurial glia |
| Genetic reagent (D. melanogaster) | R54H02-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_45784 | Cortex glia |
| Genetic reagent (D. melanogaster) | R77A03-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_39944 | Cortex glia |
| Genetic reagent (D. melanogaster) | R86E01-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_45914 | Astrocytes |
| Genetic reagent (D. melanogaster) | R56F03-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_39157 | Neuropile ensheathing glia |
| Genetic reagent (D. melanogaster) | R75H03-Gal4 | (Kremer, et al., 2017) | RRID:BDSC_39908 | Tract ensheathing glia |
| Genetic reagent (D. melanogaster) | Moody-Gal4 | Gift from Dr. Andrea Brand | ||
| Genetic reagent (D. melanogaster) | 46F-Gal4 | A gift from Dr. Vanessa Auld | ||
| Genetic reagent (D. melanogaster) | Gli-Gal4 | A gift from Dr. Vanessa Auld | ||
| Genetic reagent (D. melanogaster) | Nrv2-Gal4 | A gift from Dr. Vanessa Auld | ||
| Genetic reagent (D. melanogaster) | Alarm-Gal4 | RRID:BDSC_67031 RRID:BDSC_67032 |
||
| Genetic reagent (D. melanogaster) | NP2222-Gal4 | (Hayashi, et al., 2002) | RRID:DGGR_112830 | |
| Genetic reagent (D. melanogaster) | Elav-Gal80 | (Yang, et al., 2009) | ||
| Genetic reagent (D. melanogaster) | UAS-dStim-RNAi | Verified in (Petersen, Wolf, & Smyth, 2020) | RRID:BDSC_27263 | dStimRNAi#1 |
| Genetic reagent (D. melanogaster) | UAS-dStim-RNAi | RRID:BDSC_41759 | dStimRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-dStim-RNAi | RRID:BDSC_51685 | dStimRNAi#3 | |
| Genetic reagent (D. melanogaster) | UAS-dStim-RNAi | RRID:BDSC_52911 | dStimRNAi#4 | |
| Genetic reagent (D. melanogaster) | UAS-dStim-RNAi | RRID:FlyBase_FBst0478081 | dStimRNAi#5 | |
| Genetic reagent (D. melanogaster) | UAS-Orai-RNAi | RRID:FlyBase_FBst0450445 | OraiRNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-Orai-RNAi | Verified in (Petersen, et al., 2020) | RRID:BDSC_53333 | OraiRNAi#2 |
| Genetic reagent (D. melanogaster) | UAS-SERCA-RNAi | RRID:FlyBase_FBst0465735 | SERCARNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-SERCA-RNAi | RRID:FlyBase_FBst0479267 | SERCARNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-IP3R-RNAi | Verified in (Kohn et al., 2015) | RRID:FlyBase_FBst0451656 | ItpRRNAi#1 |
| Genetic reagent (D. melanogaster) | UAS-IP3R-RNAi | Verified in (Kohn, et al., 2015) | RRID:FlyBase_FBst0470335 | ItpRRNAi#1 |
| Genetic reagent (D. melanogaster) | UAS-IP3R-RNAi | RRID:BDSC_51686 | ItpRRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS- IP3R -RNAi | RRID:BDSC_51795 | ItpRRNAi#3 | |
| Genetic reagent (D. melanogaster) | UAS- RyR -RNAi | RRID:BDSC_65885 | RyRRNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS- RyR -RNAi | RRID:BDSC_31540 | RyRRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS- RyR -RNAi | RRID:BDSC_29445 | RyRRNAi#3 | |
| Genetic reagent (D. melanogaster) | UAS-TRPA | (Y. V. Zhang, et al., 2017) | ||
| Genetic reagent (D. melanogaster) | UAS-ChR2XXL | (Dawydow et al., 2014) | ||
| Genetic reagent (D. melanogaster) | UAS-Shits | RRID:BDSC_66600 | Shits | |
| Genetic reagent (D. melanogaster) | UAS-inx1-RNAi | RRID:BDSC_55601 | Inx1RNAi | |
| Genetic reagent (D. melanogaster) | UAS-inx2-RNAi | RRID:BDSC_42645 | Inx2RNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-inx2-RNAi | RRID:BDSC_80409 | Inx2RNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-inx1DN | Gift from Dr. Andrea Brand | Inx1DN | |
| Genetic reagent (D. melanogaster) | UAS-inx2DN | Gift from Dr. Andrea Brand | Inx2DN | |
| Genetic reagent (D. melanogaster) | Tub-Gal80ts | RRID:BDSC_7018 RRID:BDSC_7019 | ||
| Genetic reagent (D. melanogaster) | UAS-IVS-mCD8::GFP | RRID:BDSC_32186 | mCD8::GFP | |
| Genetic reagent (D. melanogaster) | UAS-Esyt2::mCherry | (Kikuma, et al., 2017) | RRID:BDSC_77130 | Esyt2::mCherry |
| Genetic reagent (D. melanogaster) | C155-gal4 Elav-gal4 | RRID:BDSC_458 RRID:BDSC_8760 |
||
| Chemical compound | Dextran | Sigma Aldrich | #30024 | 1 mM |
| Chemical compound | Thapsigargin | Sigma Aldrich | #T9033 | 10μM |
| Antibody | Mouse monoclonal anti-repo | DHSB | RRID:AB_528448 | 1:50 |
| Antibody | Rat monoclonal antielav | DHSB | RRID:AB_528218 | 1:100 |
| Antibody | Rabbit polyclonal antiGFP-488 | Invitrogen | #A21311 RRID:AB_221477 |
1:500 |
| Antibody | DyLight 649 conjugated anti-HRP (horseradish peroxidase) | Jackson ImmunoResearch | #123-605-021 | 1:2000 |
| Antibody | Goat polyclonal antiMouse405 | Life technologies | #A31553 RRID:AB_221604 |
1:3000 |
| Antibody | Goat polyclonal anti-Rat555 | Invitrogen | #A21434 RRID:AB_2535855 |
1:3000 |
| Reagent | EZ-RNA II kit | Biological Industries, Israel | #20-410-100 | |
| Reagent | High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor | Thermo Scientific | AB-4374966 | |
| Reagent | Fast SYBR® Green Master Mix | Applied Biosystems | AB-4385612 | |
| Reagent | Vectashield | Vector Laboratories | RRID:AB_2336789 |