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. 2022 Apr 12;13:807125. doi: 10.3389/fphar.2022.807125

FIGURE 3.

FIGURE 3

Rutaecarpine regulates oxidative damage in TBI mice neurons through activation of the PGK1-NRF2 signaling pathway. (A) Expressions of PGK1-NRF2 pathway-related proteins NRF2, Nucleus-NRF2, HO-1 and PGK-1 in cerebral cortex. Relative expressions of (B) NRF2, (C) Nucleus-NRF2, (D) HO-1 and (E) PGK-1. (F) Representative images of PGK1 and DAPI immunofluorescence staining. (G) Relative intensity of immunofluorescence of PGK1 and NeuN. (H) With the treatment of CHX, rutaecarpine further reduced the expression of PGK1. (I) The relative expression of PGK1 was presented in histograms. Lamin B was used as an internal control for Nucleus-NRF2, and GAPDH was used as an internal control for remaining proteins in (A). Compared with the control group, #p < 0.05 and ##p < 0.01; compared with the model group, *p < 0.05 and **p < 0.01.