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. 2022 Apr 12;13:807125. doi: 10.3389/fphar.2022.807125

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Copyright © 2022 Xu, Li, Liu, Lu, Ling and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rutaecarpine activates the NRF2 pathway in PC12 cells in response to H2O2-induced oxidative stress. (A) Expressions of NRF2 pathway-related proteins NRF2, Nucleus-NRF2 and HO-1 in PC12 cells. Relative expressions of (B) NRF2/GAPDH, (C) Nucleus-NRF2/Lamin B and (D) HO-1/GAPDH. (E) Represent images of NRF2 and DAPI immunofluorescence staining. (F) Relative intensity of immunofluorescence of NRF2. Lamin B was used as an internal control for Nucleus-NRF2, and GAPDH was used as an internal control for remaining proteins (A). Compared with the control group, #p < 0.05 and ##p < 0.01; compared with the model group, *p < 0.05 and **p < 0.01.