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. 2022 Apr 21;9:858261. doi: 10.3389/fnut.2022.858261

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Copyright © 2022 Wang, Yuan, Wang, Wang, Zou, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of TBs-C on lung cancer cell proliferation, apoptosis, and cell cycle in vitro. (A) The CCK-8 assay was performed to determine the viability of A549 and H2030 cell lines after 24 and 48 h of treatment with different doses of TBs-C. (B) The capacity of A549 and H2030 cell lines to form clones was examined at varying concentrations of TBs-C. (C) The analysis of the cell cycle by flow cytometry after a 24 h treatment with TBs-C is depicted. (D) Quantitative results of the cell cycle analysis. (E) Evaluation of the apoptosis of cells treated for 24 h of TBs-C by AnnexinV-7AAD flow cytometry. (F) Quantitative analysis of cell apoptosis, *p < 0.05, **p < 0.01.