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. 2022 Apr 21;9:858261. doi: 10.3389/fnut.2022.858261

FIGURE 5.

FIGURE 5

Inhibition of autophagy overcomes the antitumor effect of TBs-C in NSCLC cells. (A) Cells pretreated with or without 3-MA were treated with TBs-C (60 μg/mL) for 24 h, and the protein levels of LC3 and p62 were detected by Western blotting. (B) Cells pretreated with or without 3-MA were treated with TBs-C for 24 h, and the cell viabilities were determined by the CCK-8 assay. (C) Cells transfected with si-NC and si-ATG5 were treated with TBs-C (60 μg/mL) for 24 h, and the protein levels of LC3 and p62 were detected by Western blotting. (D) Cells transfected with si-NC and si-ATG5 were treated with TBs-C for 24 h, and the cell viabilities were determined by CCK-8 assay, *p < 0.05, **p < 0.01.