Table 3.

Primers and probe sequences and final concentrations of the oligonucleotides targeting the E-gene^a, N-gene^b, and human RP-gene^c by LDT^d RT-PCR^e.

Oligonucleotide name	Sequence (5’-3’)	Final concentration (nM)
E_Sarbeco_F	ACAGGTACGTTAATAGTTAATAGCGT	500
E_Sarbeco_R	ATATTGCAGCAGTACGCACACA	400
E_Sarbeco_P	LC610f-ACACTAGCCATCCTTACTGCGCTTCG-BBQg	150
N2_CDC_F	TTACAAACATTGGCCGCAAA	400
N2_TibMol_R1	AAGGTGTGACTTCCATGCCA	400
N2_CoV2_P	FAMh- ACAATTTGCCCCCAGCGCTTCAG-BBQ	150
H_RnaseP_F	AGATTTGGACCTGCGAGCG	100
H_RnaseP_R	GAGCGGCTGTCTCCACAAGT	100
H_RnaseP_P	Cy5i- TTCTGACCTGAAGGCTCTGCGCG-BBQ	125

^aE-gene: envelope gene.

^bN-gene: nucleocapsid protein gene.

^cRP-gene: RNase P gene.

^dLDT: laboratory-developed test.

^eRT-PCR: Reverse Transcription Polymerase Chain Reaction.

^fLC610: LightCycler Red 610 fluorophore.

^gBBQ: BlackBerry Quencher.

^hFAM: 6-carboxyfluorescein fluorophore.

ⁱCy5: cyanine fluorophore.