Measurement of apiin, 3′-methoxyapiin and apigenin-7-O-β-d-glucopyranoside levels by HPLC – method validationa.
| Chemical | Calibration curve | Correlation factor (r2) | Linearity (mg mL−1) | Precision RSD (%) | Repeatability RSD (%) | Recovery (%) | Limit of quantitation (μg mL−1) |
|---|---|---|---|---|---|---|---|
| Apiin | Y = 27 365 340.54x + 214 217.29 | 0.9998 | 0.24–0.84 | 0.14 | 0.11 | 98.7 | 10 |
| 3′-Methoxyapiin | Y = 17 026 768.87x + 209 822.11 | 0.9997 | 0.26–0.91 | 0.12 | 0.65 | 98.29 | 15 |
| Apigenin-7-O-β-d-glucopyranoside | Y = 64 779 573.78x + 154 434.71 | 0.9998 | 0.22–0.77 | 0.1 | 1.02 | 97.69 | 6.5 |
a
Samples were analyzed by HPLC, and standard curves were plotted to determine the concentration of each component. Results of 6 validation methods are expressed as mean values (n = 3), and the standard error of the mean (SEM) was <5%.