Extended Data Fig. 3. Immunohistochemical analyses reveal similar cell composition in iCtrl and Mut fusion organoids.

a, Day ~100 iCtrl and Mut Cx+GE fusion organoids have comparable numbers of GAD65⁺ positive cells in both the GE and Cx end (quantification in Fig. 3c). b, Both unfused Mut and unfused iCtrl day ~100 GE organoids contain multiple interneuron subtypes including CALRETININ, CALBINDIN, and SOMATOSTATIN (SST) expressing cells. c, Mut and iCtrl Day ~100 GE and Cx organoids also contain GFAP⁺ astrocytes. All images are representative examples from 3 or more independently imaged sections. See Supplementary Table 4 for additional details.