Extended Data Fig. 8. Spatially restricted microcircuit clusters and fewer synchronous events in MECP2 Mut Cx+GE organoids.

a, Pooled data for neuronal clusters derived here using Ca²⁺ activity correlations, reveal spatially restricted (smaller) microcircuit clusters with fewer average neurons per cluster in Mut compared to iCtrl. b, Pooled data of synchronous events demonstrates significantly fewer events (but with each event having a significantly higher amplitude, see Fig. 3) in Mut compared to iCtrl. Synchronous events have similar overall duration in both conditions (n = 6 for iCtrl, n = 7 for Mut and represents independently generated organoids, *P = 0.0436 for Pairwise Distances, *P = 0.0203 for Cluster Circumference, *P = 0.0321 for Cluster Area, **P = 0.0089 for Neurons per Cluster, and *P = 0.0180 for Number of Synchronized Transients). Plots display the full distribution of individual data points with dotted lines to indicate the median and quartile values. Following a normality test, statistical significance was determined using a two-sided Mann-Whitney U-test.