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. 2022 Feb 9;189(1):49–65. doi: 10.1093/plphys/kiac048

Figure 7.

Figure 7

Enzyme assays verified the PLPP function of the At2g33255 encoded protein. A, Time course of phosphate release from PLP (0.2 mM). B, Phosphate release is dependent on the active enzyme (+E), presence of PLP (0.2 mM), and Mg2+. Each assay time was 30 min. C, Michaelis–Menten curve indicating substrate saturation of enzyme activity, the inset shows a corresponding Lineweaver–Burk analysis indicating an apparent Km for PLP of 30 µM and a Vmax of 0.123 µmol mg protein−1 min−1. Each assay time was 30 min. Shown are mean values of four replicates ± se.