Table 1.

Enzymatic activity of At2g33255 on different putative substrates

Tested substrate	Phosphate release as %
6-phosphogluconic acid	n.d.
d-Fructose-6-phosphate	n.d.
d-Fructose-1,6-bisphosphate	n.d.
d-Glucose-6-phosphate	3.5
α-d-glucose-1-phosphate	n.d.
Glyceraldehyde 3-phosphate	n.d.
3-Phosphoglyceric acid	6.5
Dihydroxyacetone-phosphate	9.0
Phosphoenolpyruvate	n.d.
Adenosine triphosphate	30.5
Adenosine diphosphate	1.8
Adenosine monophosphate	3.2
PLP	100.0

Notes: Assays were performed with 1 mM substrate and 6.25 µg of the recombinant phosphatase per 0.5 mL. A colorimetric change of the assay mix indicated release of phosphate after 30 min of incubation at 23°C. n.d., not detectable ≤ 1%.