Table 2.
Altered amino acid contents of had1-2, had1-3, er-ant1, and had1-2 x er-ant1 mutants when compared with wt plants
Amino acid | had1-2 (%) | had1-3 (%) | er-ant1 (%) | had1-2 x er-ant1 (%) |
---|---|---|---|---|
Asp | 95 | 98 | 12*** | 93* |
Glu | 104 | 93 | 24*** | 82* |
Glycine | 158 | 102 | 6,847*** | 6363*** |
Ala | 115 | 104 | 20*** | 96 |
Arg | 109 | 122 | 847*** | 124 |
Notes: Plants were grown at 2,000 ppm CO2 for 4 weeks and subsequently shifted to ambient air for 5 d. Samples were taken on the fifth day, 5 h after onset of light. Shown are the mean values of four individual replicates. The obtained concentrations were normalized to the respective wt concentration (set to 100%). Asterisks indicate the significance levels between wt and mutants according to a Student’s t test.
P < 0.05,
P < 0.001.