Figure 6.

Effects of Tip110 expression on NF-κB responsive gene expression. 293T were transfected with 50 nM of Si-Ctrl or Si-Tip110, cultured for 24 hr, treated with TNF-α (10 ng/ml) for 0, 2, 4 or 24 hr, and harvested for total RNA isolation and qRT-PCR for TNF-α, IL-6, and IL-8 mRNA expression. The relative mRNA expression was normalized to β-actin and expressed as fold increases over those in Si-Ctrl-transfected and untreated cells. The data were mean ± from triplicate samples. *P < 0.05.