Figure 8.

The proliferation, migration or tube formation of L929 and HUVEC are produced by culture medium (CM) from AGEs-ARF-MEL-induced macrophages, AGEs-induced macrophages, and unstimulated macrophages. (A) Schematic depicting the process of paracrine signaling from the AGE-ARF-MEL-treated macrophage conditioned medium. (B) Representative flow cytometry results of the CD86+ and CD206+ populations in macrophages treated with 100 ng/ml LPS (M1 polarized), 40 ng/ml IL-4 (M2 polarized), AGEs (100 μg/ml), ARF 1% and MEL 20 μM compared with the untreated control. *P < 0.05 and **P < 0.01 compared with the control group. n = 5–8. Error bars represent SDs. (C) The proliferation rate of HUVEC and L929 cells pretreated with different CMs was determined using the CCK-8 kit (one-way ANOVA). **P < 0.01 compared with the control-CM group or AGEs group. N = 5. Error bars represent SDs. (D) A tube formation assay of HUVEC and scratch healing assay of L929 were treated with different CMs for 12 h, scale bar of tube formation image, 100 μm; scale bar of scratch healing assays image, 250 μm. (E) The tube length and scratch area were quantified using image analysis (one-way ANOVA). **P < 0.01 compared with the A-AM group. n = 3. Error bars represent SDs