Table III.

Transcription factors reported to be expressed in bona fide hPGCs and in vitro derived hPGCLCs.

Bona fide hPGCs			In vitro derived hPGCLCs			Functions in reproduction based on Mouse Genome Informatics (MGI)
Gkountela et al. (2013)	Gkountela et al. (2015)	Chen et al. (2018)	Sasaki et al. (2015)	Chen et al. (2018)	Sybirna et al. (2020)
ALX4	ALX4	ALX4			ALX4	Male sterility
BNC1	BNC1	BNC1			BNC1	Required for testis development
		CDX1	CDX1	CDX1	CDX1
		DLX5		DLX5	DLX5
DMRT1	DMRT1	DMRT1			DMRT1	Male sterility, disorganized seminiferous tubules
DMRTC2		DMRTC2				Male sterility
EMX2	EMX2	EMX2				Bipotential gonad marker
ESX1	ESX1	ESX1				Role in spermatogenesis
			GATA2/3/4	GATA2/3/4	GATA2/3/4
	HOXA2	HOXA2
HOXA3/4/5/7/9	HOXA3/4/5/7/9	HOXA3/4/5/7/9
	HOXB3/4/5	HOXB3/4/5
HOXB7		HOXB7
HOXC4	HOXC4	HOXC4
HOXC9		HOXC9
HOXD3		HOXD3
HOXD9	HOXD9	HOXD9
			IRX1	IRX1	IRX1
	IRX4	IRX4
			IRX6	IRX6	IRX6
KLF2	KLF2
			KLF4	KLF4	KLF4
			LHX1		LHX1
LHX2		LHX2
			MSX2	MSX2	MSX2
NR2F2	NR2F2		NR2F2
	OSR2	OSR2
PAX5		PAX5				PGCs development
PAX8	PAX8	PAX8				Male infertility
RNF17	RNF17	RNF17			RNF17	Testis specific; regulates piRNA maturation
			RUNX3	RUNX3	RUNX3
SIX1		SIX1
			SOX15	SOX15	SOX15
			SOX17	SOX17	SOX17
			T	T	T
TBX2		TBX2
		TBX3	TBX3	TBX3	TBX3
TBX5		TBX5
			TCL1A	TCL1A
			TFAP2C	TFAP2C	TFAP2C
	TFCP2L1	TFCP2L1	TFCP2L1	TFCP2L1	TFCP2L1
TLX2		TLX2
ZEB1	ZEB1

PiRNA, Piwi-interacting RNA.