Fig 2. Time-of-addition analysis.

(a) The schematic diagram of time-of addition. Approximately 8×10⁴ Vero-E6 cells were seeded per well of a 24-well plate. After incubation for 24 h, the cells were infected with the SARS-CoV-2 virus at an MOI of 0.05. Berbamine hydrochloride was added to the infected cells at a final concentration of 10 μM at indicated time points. At 12 h p.i., the cell culture fluids were harvested for qRT-PCR assay (b). Data was expressed as mean ± standard deviation and shown are representative results of two independent experiments with two technical replicas per experiment. Error bars represents standard deviation.