Figure 3.

Co-staining of ferroptosis-related proteins with cell type specific markers. (A–C) Double immunofluorescence with ACSL4 and GFAP in a primary GBM shows several cells with co-expression (yellow arrow). (D–F) Double immunofluorescence with GPX4 and GFAP shows co-expression in many cells in a primary GBM (yellow arrow). Additionally, one cell expressing only GPX4 (green arrow) is marked. (G–I) Double immunofluorescence with ACSL4 and Iba1 in a primary GBM shows several Iba1⁺ cells which mostly do not express ACSL4 (red arrow: Iba1-positive cell lacking ACSL4 expression; green arrow: ACSL4-positive non-microglial cell). (J–L) Double immunofluorescence with GPX4 and Iba1 in a relapse GBM. Most cells express either GPX4 (green arrow) or Iba1 (red arrow). Scale bars all 20 μm.