Figure 6.

Co-staining of both ferroptosis-related proteins in pairs of primary and recurrent GBM. (A–C) Double immunofluorescence with ACSL4 and GPX4 in a primary GBM (patient A) shows cells expressing either ACSL4 (red arrow) or GPX4 (green arrow). (D–F) Double immunofluorescence with ACSL4 and GPX4 in a relapse GBM (patient A) shows several cells with co-expression (yellow arrow), as well as only ACSL4⁺ cells (red arrow) and GPX4⁺ cells (green arrow). (G–I) Double immunofluorescence with ACSL4 and GPX4 in a primary GBM (patient B) shows ACSL4⁺ cells (red arrows) and GPX4⁺ cells (green arrow). Some cells express both ACSL4 and GPX4 (yellow arrow). (J–L) Double immunofluorescence with ACSL4 and GPX4 in a relapse GBM (patient B). Whilst many cells show co-expression (yellow arrow), one cell only expressing ACSL4 is marked (red arrow). Scale bars all 20 μm.