Table 2.
Used antibodies.
| Antibody | Company | Clone | Host species | Dilution |
|---|---|---|---|---|
| Anti-ACSL4 | Santa Cruz, USA | Monoclonal, clone IgG2b | mouse | IHC: 1/100 IF: 1/20 |
| Anti-GPX4 | Abcam, UK | Monoclonal, clone EPNCIR144 | rabbit | IHC: 1/3000 IF: 1/1000 |
| Anti-ALDH1A3 | Thermo Fisher Scientific, USA | polyclonal | rabbit | IHC: 1/1000 |
| Anti-FSP1 | developed in house | IgG2 monoclonal antibody raised against a N-terminal peptide of hXCT, clone 3A12-1-1 | rat | IHC: undiluted |
| 2nd antibody, anti-rabbit | Vector Laboratories, USA | IgG | rabbit | IHC: 1/400 |
| 2nd antibody, anti-mouse | Vector Laboratories, USA | IgG | mouse | IHC: 1/400 |
| 2nd antibody, anti-rat | Vector Laboratories, USA | IgG | rat | IHC: 1/400 |
| anti-GFAP | Dako, USA | monoclonal | mouse | IF: 1/50 |
| anti-GFAP | Dako, USA | polyclonal | rabbit | IF: 1/500 |
| anti-Iba1 | Abcam, UK | monoclonal | mouse | IF: 1/500 |
| anti-Iba1 | Wako, USA | polyclonal | rabbit | IF: 1/500 |
| 2nd antibody | Invitrogen/Thermo Fisher Scientific, USA | Polyclonal, (Alexa Fluor 568/488) | mouse | IF: 1/2000 |
| 2nd antibody | Invitrogen/Thermo Fisher Scientific, USA | Polyclonal, (Alexa Fluor 568/488) | rabbit | IF: 1/2000 |