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. 2021 Nov 19;24(5):726–738. doi: 10.1093/neuonc/noab264

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© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Zebrafish xenografted with patient-derived cells (PDCs) can be monitored in real-time using automatic imaging and image analysis. (A) Experiment workflow. High-throughput injection of GFP-tagged PDCs can be monitored in real-time in six 96-well plates using the IncuCyte S3. (B) Pixel-wise semantic segmentation for tumor localization and post-classification of image types. Left panel: Bright Field; Middle panel: Green Fluorescence; Right panel: post-classification. (C and D) Confusion matrices of post-segmentation image classifications for precision (C) and sensitivity (D).