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. 2022 Feb 19;9:uhac038. doi: 10.1093/hr/uhac038

Figure 4.

Figure 4

Brp participates in regulating cucurbitacin biosynthesis. a Schematic diagram showing the constructs used in the transient transcriptional activity assays. b, cBrp activates Bi expression in a tobacco leaf assay system for melon (b) and watermelon (c). Values are mean ± standard deviation from four biological replicates. d GFP signals detected in hairy roots of CmeBrp-overexpressing and CmeBrp knockout lines. Scale bar = 1 mm. e Sequence analysis of CmeBrp in two knockout lines. A total of 25 colonies were sequenced in CmeBrp-cr lines. Mutant types and numbers are showed at right. f Content of CuB in WT, CmeBrp knockout, and CmeBrp overexpressing lines. g Relative expression of CmeBrp in WT, CmeBrp knockout, and CmeBrp-overexpressing lines. h–k Relative expression of four key CuB biosynthetic genes, CmeBi (h), Cme890 (i), Cme180 (j), and CmeACT (k), in WT, CmeBrp knockout, and CmeBrp-overexpressing lines. Transcript levels were measured by RT–qPCR. Values are represented as mean ± standard deviation from three biological replicates. **P <.01, Student’s t-test.