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. 2022 Apr 19;50(8):4484–4499. doi: 10.1093/nar/gkac253

Figure 6.

Figure 6.

DNase I footprinting of PvpsL transcription complexes containing WT VpsR or VpsRren on nontemplate (A) or template (B) DNA. GA corresponds to G + A ladder. Reactions contained VpsR WT, VpsRren or VpsRren pretreated with Ac∼P, 50 μM c-di-GMP and/or RNAP as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1 TSS. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex containing RNAP, DNA, c-di-GMP, and either VpsRren pretreated with Ac∼P or WT VpsR are depicted as rectangles and horizontal arrows, respectively.