Figure 7.
Pathway for the biosynthesis of Ac∼P and vpsL-lux gene reporter assays in V. cholerae WT and ΔackA1/2Δpta. (A) Ac∼P is generated by the enzyme Pta and is degraded by the two enzymes AckA1 and AckA2 in V. cholerae. (B) Expression from pBH625 (vpsL-lux) is shown for either V. cholerae WT (black) or ΔackA1/2 Δpta (gray) containing pBH625 (vpsL-lux) and with overexpression of either the DGC QrgB or QrgB* to generate high or unaltered intracellular c-di-GMP, respectively. Error bars indicate standard deviation of six independent cultures and significance was determined using a multiple unpaired t-test with a two-stage Benjamini, Krieger and Yekutieli correction (**P < 0.01).