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. 2021 Dec 1;50(8):4201–4215. doi: 10.1093/nar/gkab1150

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — In cellulo degradation of total RNA after organismal death. (A) Workflow: mice were sacrificed by cervical dislocation. RNA from liver and brain tissue was isolated at different time points post mortem. Additionally, the effect of shock freezing was assessed from RNA isolated immediately after dissection. (B) Representative TapeStation Profiles of total RNA from brain (light brown) and liver (dark brown), respectively, at the time points indicated in A. (C) RNA integrity (RIN^e) values of liver and brain RNA plotted over time (mean ± SD, n = 4 biological replicates, n.s. = not significant, *P< 0.05, ****P< 0.0001, unpaired t-test, compared to 0 h without freezing). (D) Relative Quantification of the rRNA marker m^6,6A by LC–MS/MS shows elevated m^6,6A levels in tRNA fraction of liver and brain RNA in a time-depentend manner. Mean ± SD, n = 4 biological replicates.