Table 1.
Details of LC−MS/MS detection of modified nucleosides
| Dynamic MRM | ||||||||
|---|---|---|---|---|---|---|---|---|
| Compound name | Precursor ion | Product ion | Fragmentor | Collision energy | Cell accel. voltage | Ret time (min) | ΔRet time | Polarity |
| Am | 282 | 136.0 | 92 | 13 | 2 | 16.4 | 5 | Positive |
| Am 13C | 293 | 141.0 | 92 | 13 | 2 | 16.4 | 5 | Positive |
| Cm | 258 | 112.1 | 60 | 9 | 2 | 10.0 | 5 | Positive |
| Cm 13C | 268 | 116.1 | 60 | 9 | 2 | 10.0 | 5 | Positive |
| D | 247 | 115.0 | 80 | 10 | 2 | 3.9 | 5 | Positive |
| D 13C | 255 | 119.0 | 80 | 10 | 2 | 3.9 | 5 | Positive |
| Gm | 298 | 152.0 | 72 | 5 | 2 | 13.2 | 5 | Positive |
| Gm 13C | 309 | 157.0 | 72 | 5 | 2 | 13.2 | 5 | Positive |
| I | 269 | 137.0 | 76 | 5 | 2 | 10.5 | 5 | Positive |
| I 13C | 279 | 142.0 | 76 | 5 | 2 | 10.5 | 5 | Positive |
| i6A | 336 | 204.0 | 80 | 15 | 2 | 22.3 | 5 | Positive |
| i6A 13C | 351 | 214.0 | 80 | 15 | 2 | 22.3 | 5 | Positive |
| m1A | 282 | 150.0 | 92 | 17 | 2 | 5.6 | 6 | Positive |
| m1A C13 | 293 | 156.0 | 92 | 17 | 2 | 5.6 | 6 | Positive |
| m1G | 298 | 166.0 | 82 | 9 | 2 | 13.0 | 5 | Positive |
| m1G C13 | 309 | 172.0 | 82 | 9 | 2 | 13.0 | 5 | Positive |
| m2,2G | 312 | 180.0 | 82 | 9 | 2 | 15.4 | 5 | Positive |
| m2,2G 13C | 324 | 187.0 | 82 | 9 | 2 | 15.4 | 5 | Positive |
| m6,6A | 296 | 164.1 | 102 | 17 | 2 | 18.9 | 5 | Positive |
| m6,6A 13C | 308 | 171.0 | 102 | 17 | 2 | 18.9 | 5 | Positive |
| m2A | 282 | 150.1 | 92 | 17 | 2 | 16.9 | 5 | Positive |
| m2A 13C | 293 | 156.0 | 92 | 17 | 2 | 16.9 | 5 | Positive |
| m2G | 298 | 166.1 | 82 | 9 | 2 | 13.6 | 5 | Positive |
| m2G 13C | 309 | 172.0 | 82 | 9 | 2 | 13.6 | 5 | Positive |
| m3C | 258 | 126.0 | 40 | 9 | 2 | 6.8 | 6 | Positive |
| m3C C13 | 268 | 131.0 | 40 | 9 | 2 | 6.8 | 6 | Positive |
| m3U | 259 | 127.0 | 76 | 5 | 2 | 12.6 | 6 | Positive |
| m3U 13C | 269 | 132.0 | 76 | 5 | 2 | 12.6 | 6 | Positive |
| m5C | 258 | 126.1 | 40 | 9 | 2 | 9.5 | 5 | Positive |
| m5C 13C | 268 | 131.0 | 40 | 9 | 2 | 9.5 | 5 | Positive |
| m5U | 259 | 127.0 | 76 | 5 | 2 | 11.2 | 6 | Positive |
| m5U 13C | 269 | 132.0 | 76 | 5 | 2 | 11.2 | 6 | Positive |
| m6A | 282 | 150.1 | 92 | 17 | 2 | 17.1 | 5 | Positive |
| m6A 13C | 293 | 156.0 | 92 | 17 | 2 | 17.1 | 5 | Positive |
| m7G | 298 | 166.1 | 82 | 9 | 2 | 9.7 | 6 | Positive |
| m7G C13 | 309 | 172.0 | 82 | 9 | 2 | 9.7 | 6 | Positive |
| mcm5U | 317 | 185.0 | 66 | 5 | 2 | 13.2 | 5 | Positive |
| mcm5U 13C | 329 | 192.0 | 66 | 5 | 2 | 13.2 | 5 | Positive |
| ms2i6A | 382 | 250.0 | 80 | 15 | 2 | 22.4 | 5 | Positive |
| ms2i6A 13C | 398 | 261.0 | 80 | 15 | 2 | 22.4 | 5 | Positive |
| Ψ | 245 | 209.0 | 81 | 5 | 2 | 4.2 | 5 | Positive |
| Ψ C13 | 254 | 218.0 | 81 | 5 | 2 | 4.2 | 5 | Positive |
| Q | 410 | 163.0 | 80 | 15 | 2 | 11.6 | 6 | Positive |
| Q 13C | 427 | 170.0 | 80 | 15 | 2 | 11.6 | 6 | Positive |
| s2C | 260 | 128.0 | 40 | 10 | 2 | 9.2 | 6 | Positive |
| s2C 13C | 269 | 132.0 | 40 | 10 | 2 | 9.2 | 6 | Positive |
| s2U | 261 | 129.0 | 66 | 5 | 2 | 11.1 | 6 | Positive |
| s2U 13C | 270 | 133.0 | 66 | 5 | 2 | 11.1 | 6 | Positive |
| t6A | 413 | 281.0 | 80 | 15 | 2 | 15.2 | 8 | Positive |
| t6A 13C | 428 | 291.0 | 80 | 15 | 2 | 15.2 | 8 | Positive |
| Tm | 273 | 127.0 | 66 | 5 | 2 | 14.9 | 5 | Positive |
| Tm 13C | 286 | 134.0 | 66 | 5 | 2 | 14.9 | 5 | Positive |
| Um | 259 | 113.0 | 66 | 5 | 2 | 12.1 | 5 | Positive |
| Um 13C | 269 | 117.0 | 66 | 5 | 2 | 12.1 | 5 | Positive |