Table 2.

Absolute quantification of RNA modifications. The numbers correspond to the modification content of species in Figure 1B. Only those 17 modifications for which SILIS and external calibration standards were available could be quantified in abolsute terms, and for lack of a defined sequence, results arenormalized to the UV signal of adenosine [%]. bdl = below detection limit. * = quantification of this peak has exceeded calibration range.

	tRNA #2	tRNA #1	mRNA #1	28S rRNA #2	28S rRNA #1	18S rRNA #2	18S rRNA #1	Input #2	Input #1
m3U	0.044	0.035	bdl	0.273	0.214	0.015	0.024	0.050	0.055
Um	2.349	1.913	bdl	3.102	5.258	2.600	2.603	1.587	1.594
m6,6A	0.013	0.010	0.006	0.154	0.490	0.950	1.469	0.278	0.451
Cm	0.523	0.426	0.017	0.887	0.594	0.585	0.444	0.684	1.533
Am	0.034	0.024	0.043	2.754	2.070	2.555	2.017	1.915	2.949a
Gm	1.335	1.066	0.069	2.949	1.829	1.907	1.539	1.876	2.328a
m6A	0.649	0.498	0.229	0.230	0.240	0.173	0.319	0.306	0.304
m3C	0.602	0.466	2.0E-04	0.006	0.032	0.006	0.057	0.090	0.202
I	0.884	0.670	bdl	bdl	0.057	bdl	0.096	0.217	0.167
i6A	0.204	0.149	1.5E-04	0.002	0.011	0.002	0.020	0.031	0.036
m5C	7.484	6.279	0.011	0.354	0.632	0.114	0.783	1.355	*2.273
m7G	4.664	4.070	0.149	0.097	0.349	0.358	0.666	0.880	1.026
t6A	0.703	0.590	bdl	0.008	0.046	0.008	0.075	0.121	0.138
m1G	2.975	2.500	0.001	0.032	0.170	0.034	0.297	0.486	0.565
m2G	6.193	5.077	bdl	0.059	0.328	0.054	0.583	0.987	1.135
m2,2G	2.293	1.890	4.9E-04	0.020	0.125	0.020	0.222	0.360	0.454
m5U	3.083	2.529	bdl	0.041	0.195	0.038	0.303	0.482	0.609

bdl = below detection limit.

^aQuantification of this peak has exceeded calibration range.