Figure 4.

Gene coexpression conservation is associated with ortholog concordance across algorithms and can predict human-yeast functional analogs. (A) Heatmap of algorithm concordance. The majority of algorithms (9/12) make similar predictions, with outliers arising from selection biases (i.e. inclusion of only a subset of species). (B) Mean conservation for human-worm orthologs is plotted against the number of algorithms predicting the relationship. Conservation of gene neighborhoods correlates with ortholog confidence. (C) Bars show the correlation between the number of algorithms and conservation of neighborhoods for each gene pair, binned into three divergence times. Conservation correlates with ortholog confidence for pairs of species that diverged > 100MYA but not for more recently diverged species. (D) Cumulative success of human-yeast complementation is plotted as a function of gene activity conservation. Human genes with conserved gene neighborhoods are likely to compensate for loss of their yeast orthologs.