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. 2022 Apr 19;50(8):4647–4658. doi: 10.1093/nar/gkac241

Figure 5.

Figure 5.

Effect of PEG on ligation fidelity and bias for T4 DNA ligase, T7 DNA Ligase, and hLig3. The normalized ligation frequency and individual overhang fidelity was generated by SMRT sequencing of ligation reactions with 100 nM of the multiplexed four-base overhang substrate and 1.75 μM T4 DNA ligase, T7 DNA ligase or hLig3 incubated 1 h at 25°C in NEBNext® Quick Ligation Buffer (Supplementary Figures S2F–H and File S1 for raw data).The ligation frequency and ligation fidelity of overhangs are grouped by GC content. For comparison to data in standard ligation buffer, the median value is indicated by a horizontal line (red for reactions in standard ligation buffer; black for reactions in PEG-containing buffer).