Table 1.
Labels, primary sequences and molar extinction coefficients of oligonucleotides used for structural studies in the paper
| Label | Sequence | ϵ [M-1.cm-1]a | Positionb | Protein |
|---|---|---|---|---|
| TBEV-1 | AAGGUAAGGGGGGCGGUC | 194 000 | 154 | Core protein C |
| TBEV-1mut | AAGAUAAGAGGAGCGGUC | 194 900 | ||
| TBEV-1b | AAGGUAAGGGGGGCGGUCCCCCUCGA | 265 300 | 154 | Core protein C |
| TBEV-2 | UUGGAGUGGGGGCGGAUGUUGGUU | 250 700 | 2447 | Envelope / NS1 |
| TBEV-2mut | UUGGAGUGAGUGCGGAUGUUGGUU | 243 200 | ||
| TBEV-3 | AUGGUGGGCACGGAAGGAC | 201 400 | 2863 | NS1 |
| TBEV-3mut | AUGGUGAGCACGAAAGGAC | 199 500 | ||
| TBEV-4 | UCGGGGAGGGGGAGGCA | 182 400 | 7382 | NS4b |
| TBEV-4mut | UCGAGGAGUGAGAGGCA | 179 900 | ||
| TBEV-5 | CUGGGGGUAGGCGUGGUGGUU | 215 000 | 7652 | NS4b / NS5 |
| TBEV-5mut | CUGUGUGUAUGCGUGUUGGUU | 210 700 | — | — |
| TBEV-6 | CUGGGAGUUGGAACGAGGUGUGUGGUC | 280 300 | 8440 | NS5 (RNA pol) |
| TBEV-6mut | CUGAGAGUUGAAACGAGUUGUGUGGUC | 276 000 | ||
| TBEV-7 | UUGUGGGGGGAGGCUAGGAGGCGAA | 268 000 | 10653 | 3′-UTR |
| TBEV-7mut | UUGUGUAGCGAAGCUAGGAUGCGAA | 256 800 | ||
| R-psG4 | AAUGGGUGGGUGGGUGGGUAA | 230 000 |
aϵ calculated for strand using nearest-neighbor model.
bPosition of the first nucleotide in TBEV reference genome NC_001672.1.