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Rational gRNA engineering for switching CRISPR/Cas9. (A) Sequence and structure analysis of gRNA scaffold (tracrRNA demonstration). We have identified a total of three motifs with close resemblance with the units possessing affinity for MBL binding. (B) MBLs are used to introduce structural constraints into gRNAs through hydrogen bonding and stacking. Sequence modification sites are indicated in red. Red rectangles: 2-amino-1,8-naphthyridine.
