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. 2022 Apr 5;50(8):4669–4684. doi: 10.1093/nar/gkac223

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — The PAZ domains of DCL5 and DCL3 proteins determines 5′ phosphate preference for dsRNA substrates. Dicing assays by (A) AtDCL3_PAZ3, (B) AtDCL3_PAZ5, (C) OsDCL3_PAZ5 and (D) OsDCL5_PAZ3 with MonoP and TriP dsRNA substrates. Left panel: Representative gel images of dicing assays by AtDCL3_PAZ3, AtDCL3_PAZ5, OsDCL3_PAZ5 and OsDCL5_PAZ3 cleaving 38-nt substrates, MonoP and TriP. Right panel: Quantification of the proportion of cleavage from the 5′ end of sense strands (15-nt bands) in the left panel. The mean values ± SD from three independent experiments are shown. AtDCL3_PAZ5 and OsDCL3_PAZ5 prefer 5′ monophosphate, while AtDCL3_PAZ3 and OsDCL5_PAZ3 do not discriminate 5′ monophosphate from triphosphate.