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. 2022 Apr 8;50(8):4450–4463. doi: 10.1093/nar/gkac246

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Mediator and pMED1 are involved in transcription recycling in vitro. (A) Schematic of the in vitro transcription recycling assay. (B) Representative two-dimensional gels showing protein profiles bound to templates as indicated. (C) DIGE comparison of template-bound proteins. (D) Template-bound proteins identified by LC–MS/MS in recycling. (E) Selected Mediator subunits bound to templates were validated by western blot. (F) Template-bound Pol II, phosphorylated Pol II and phosphorylated MED1 were examined by western blot.