Table 1.

Steps for ANI calculation based on FastANI.

Step	Code/software
Step 1: ANI calculation	fastANI—ql list.txt—rl list.txt -o out.txt—fragLen X (list.txt: files containing list of reference/query genome files; out.txt: output file; X: fragment length)
Step 2: Data filtration	cat out.txt| awk ‘{if($3>=95) print $0}’ > 95filter.txt (ANI value cutoff: 95); cat out.txt| awk ‘{if($3≥98) print $0}’ > 98filter.txt (ANI value cutoff: 98)
Step 3: Visualization	Cytoscape