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. 2022 Mar 18;221(1):iyac045. doi: 10.1093/genetics/iyac045

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Effect of Nup60 truncations on growth and sporulation. (Left) Schematic representation of full-length Nup60 (top) and truncation mutants. Full-length Nup60 contains an N-terminal region from amino acids 1 to 188, a middle region from aa 189 to 388, and a C-terminal region from aa 389 to 539. Two helical regions in the N-terminal fragment that help anchor Nup60 to the nuclear envelope are highlighted; 4 FxF repeats in the C-terminal region are shown as black bars. (Right) Table summarizing the effect of the NUP60 truncations growth and sporulation in either an NDJ1 or ndj1 strain background. Growth was assessed by streaking wild-type and nup60 strains onto YPD plates and comparing colony size after 48 h. Poorly growing strains formed smaller colonies with a nibbled morphology (Supplementary Fig. 3a). All strains in the growth column are isogenic to the wild-type (NUP60) strain: NUP60(1–539) (SBY1903), nup60Δ (SBY5217), nup60(1–388) (SBY6423), nup60(189–539) (SBY6426), nup60(Δ189–388) (SBY6429), and nup60(189–388) (SBY6420). All strains in the NDJ1 sporulation column are isogenic to the wild-type NUP60(1–539) strain (SBY1903): nup60Δ (SBY5217), nup60(1–388) (SBY6423), nup60(189–388) (SBY6420), nup60(189–539) (SBY6426), and nup60(Δ189–388) (SBY6429). All strains in the ndj1Δ sporulation column are isogenic to the NUP60(1–539) strain (SBY1904): nup60Δ (SBY5222), nup60(1–388) (SBY6296), nup60(189–388) (SBY6293), nup60(189–539) (SBY6299), and nup60(Δ189–388) (SBY6302).