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. 2022 Mar 18;221(1):iyac045. doi: 10.1093/genetics/iyac045

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Effect of nup60(189–388) on the kinetics of nuclear division. a) Epistasis analysis of nup60(189–388) and nup2Δ. Synchronized cells cultured in liquid SPM were removed for analysis at the indicated times after initial transfer. At least 200 cells were analyzed for the presence of either one or more than one DAPI-staining body. All strains are isogenic to the wild-type strain: wild-type (SBY1903) nup60(189-388) (SBY6420), nup2Δ (SBY3945), and nup60(189-388) nup2Δ (SBY6432). A representative time course is shown for 4 strains tested in duplicate on the same day. b) Epistasis analysis of nup60(189–388) and ndj1Δ. A time course experiment was performed as described in (a) using isogenic strains: WT (SBY1903), nup60(189–388) (SBY6420), nup2Δ (SBY3945), ndj1Δ (SBY1904), nup60(189–388) ndj1Δ (SBY6293), and nup2Δ ndj1Δ (SBY3983). A representative time course is shown for 6 strains tested in duplicate on the same day. c) Sporulation counts of samples from the time course depicted in (a). For each sample, at least 200 cells were analyzed under white light for the presence of 2 or more spores. d) Sporulation counts of samples from the time course depicted in (b). e) Images of DAPI-stained samples of wild type, nup2Δ ndj1Δ, and nup60(189–388) ndj1Δ strains after 24 h in sporulation media. The top panels show DAPI fluorescence, and the bottom panels show bright-field images of the same cells. The white boxes highlight examples of cells where division of the nuclear masses has taken place without spore formation.