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. 2022 Mar 18;221(1):iyac045. doi: 10.1093/genetics/iyac045

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Effect of Nup60 truncations on the localization of Nup60-GFP and MAR-mCherry to the nuclear envelope. Representative images of cells expressing wild-type MAR-mCherry and GFP fusions to the Nup60 truncations illustrated in Fig. 4 are shown. The right column is a merged image of the GFP, mCherry, and bright-field channels. All strains are isogenic to the NUP60(1–539)-GFP strain: (1–539) (SBY6254), nup60Δ (SBY6338), nup60(1–388) (SBY6344), nup60(189–388) (SBY6341), nup60(189–539) (SBY6347), and nup60(Δ189–388) (SBY6350). The intensity of the NUP60(1–539) MAR-mCherry panel was adjusted to highlight MAR-mCherry’s localization to the NE. The scale bar represents 5 µm.