Fig. 8.

Binding of Nup60-GFP to meiotic chromosomes in the absence of Nup2. a) Representative meiotic chromosome spreads from wild-type (SBY6484) or nup2Δ (SBY6487) strains expressing Nup60-GFP. Nup60(189–388)-GFP (SBY6505) was used as the negative control because chromosome binding is comparable to that of GFP, but the cells have wild-type growth. The Nup60-GFP fusions were detected using a polyclonal antibody to GFP and an Alexa Fluor 488-conjugated secondary antibody. The scale bar in the upper left panel represents 5 µm. (B) Quantitation of TCF of Nup60-GFP from spreads represented in (a). P = 0.5477, comparing Nup60-GFP in wild-type vs nup2 strain background, using a 2-tailed t-test.