TABLE 3.
Fluconazole susceptibilities of S. cerevisiae strains expressing C. albicans ERG11 genes, as determined by zone of inhibition
| Patient no. | C. albicans isolatea | Mean ± SD zone of inhibition (mm)b | ERG11 allele expressed | Amino acid substitution(s) |
|---|---|---|---|---|
| 7 | 412 (S) | 43.75 ± 1.12 | 412 | K128T |
| 2307 (R) | 43.08 ± 0.90 | 2307 | K128T | |
| 14 | 1632 (S) | 42.80 ± 1.93 | 580 | None |
| 1640 (R) | 44.18 ± 0.75 | 1640 | V437I | |
| 15 | 945 (S) | 32.50 ± 0.70c | 945 | D116E, G450E |
| 1619 (R) | 0c | 1619-1 | D116E, G450E, G307S | |
| 32.80 ± 1.35c | 1619-2 | D116E, G450E | ||
| 16 | 3107 (S) | 14.50 ± 1.24c | 3107 | Y132F |
| 3119 (R) | 15.57 ± 3.94c | 3119 | Y132F | |
| 28 | 5044 (S) | 36.81 ± 0.91c | 5044 | V437I, D446N |
| 5052 (R) | 37.56 ± 2.00c | 5052 | V437I, D446N | |
| 30 | 5106 (S) | 43.37 ± 1.50 | 5106 | V437I |
| 5108 (R) | 30.58 ± 0.79c | 5108 | V437I, G464S | |
| 42 | 1691 (S) | 42.87 ± 0.83 | 1691 | K128T |
| 3731 (R) | 0c | 3731-1 | F126L, K143R | |
| 22.33 ± 0.52c | 3731-2 | K143R | ||
| 51 | 2274 (S) | 31.55 ± 1.85c | 2274 | S405F |
| 2257 (R) | 33.16 ± 1.83c | 2257 | S405F | |
| 2339 (R) | 33.07 ± 1.64c | 2339 | S405F | |
| 59 | 3917 (S) | 42.62 ± 1.50 | 3917-1 | None |
| 32.62 ± 1.19c | 3917-2 | F449S | ||
| 4617 (R) | 21.05 ± 0.87c | 4617 | F449S, T229A | |
| 4639 (R) | 24.37 ± 1.60c | 4639 | F449S, T229A |
a
S, susceptible; R, resistant.
b
Mean diameters were obtained by measuring the zones of inhibition produced by 10 independent S. cerevisiae Leu+ transformants. The mean diameter for YKKB-13 transformed with YEp51 alone was 63 mm. The mean diameter for YKKB-13 transformed with a susceptible control was 43 mm. The mean diameter for YKKB-13 transformed with a resistant control was 0 mm.
c
P < 0.05.