Box–Behnken design matrix with three independent variables expressed in coded with absorbance values.

Run	X 1	X 2	X 3	Y (%)
1	0	0	0	2.5 ± 0.1
2	1	1	0	1.65 ± 0.05
3	0	−1	1	2.12 ± 0.09
4	−1	1	0	2.16 ± 0.06
5	−1	−1	0	1.98 ± 0.06
6	0	1	−1	2.14 ± 0.09
7	1	0	−1	2.15 ± 0.04
8	0	0	0	2.5 ± 0.1
9	1	−1	0	1.7 ± 0.1
10	1	0	−1	1.84 ± 0.04
11	0	−1	−1	1.85 ± 0.03
12	−1	0	1	1.83 ± 0.03
13	0	0	0	2.5 ± 0.1
14	0	0	0	2.5 ± 0.1
15	0	1	1	1.85 ± 0.02
16	0	0	0	2.5 ± 0.1
17	−1	0	−1	2.14 ± 0.17