Table 3.
Vector Expression in 2°CFU-S
Primary Recipient No.* | MNDeGFPSN |
LeGFPSN |
||||
---|---|---|---|---|---|---|
Expression in 1° BM | Vector† (n) |
Expression‡ | Expression in 1° BM | Vector† (n) |
Expression‡ | |
1 (8 wks) |
60% | 13/13 (3) |
13/13 | 0.4% | 13/15 (4) |
0/13 |
2 (6 mos) |
78.5% | 6/6 (2) |
6/6 | ND | ND | ND |
3 (4 mos) |
65.1% | 8/8 (3) |
8/8 | 8.4% | 8/12 (3) |
0/8 |
4 (6 mos) |
84.1% | 11/11 (4) |
11/11 | 4.4% | 12/16 (4) |
0/12 |
5 (6 mos) |
40.5% | 16/16 (4) |
16/16 | 31% | 13/13 (4) |
0/13 |
1-5 | 54/54 | 54/54 | 46/56 | 0/46 | ||
100% | 100% | 82% | 0% |
Abbreviation: (n), number of secondary transplant recipients; ND, not done.
Time after 1° transplant when 2° transplant was performed.
Number of CFU-S PCR(+) for vector DNA/number of CFU-S tested.
Number of CFU-S (+) for eGFP expression by FACS/number of CFU-S tested.