Figure 3.
Hydrogen inhibits CRC cell proliferation by decreasing pAKT/SCD1. (a) IHC assay in tumors (RKO cells) after H2 treatment and control (100x). (b) Western blot analysis showing the expression levels of p-PI3K, PI3K, AKT, pAKT, and SCD1 in RKO, SW480, and HCT116 cells before and after H2 treatment. Western blotting assays showed that the expression of SCD1 was significantly upregulated and presented a dose-dependent effect with SC79 treatment. (c) The inhibition of cell proliferation induced by H2 was reversed when cells were treated with SC79, as determined by the CCK-8 assay.